Polymorphism of Microsatellite Sequence within Protein Kinase ORFs in Phytopathogenic Fungus, Magnaporthe Grisea
نویسندگان
چکیده
Eighteen polymorphic microsatellite markers suitable for population genetic studies and protein kinase encoding genic variation measurement were developed for rice blast fungus Magnaporthe grisea. Polymorphism was evaluated by using 46 isolates collected from diverse geographical locations and rice varieties. Preliminary results indicate that each locus harbors two to
منابع مشابه
Polymorphism of Microsatellite Sequence within ABC Transporter Genes in Phytopathogenic Fungus, Magnaporthe Grisea
Thirteen polyporphic microsatellite markers suitable for population genetic structure analysis and ABC transporter and signal transduction coding genes variation measurement were developed for rice blast fungus, Magnaporthe grisea. Polymorphism was evaluated by using forty-six isolates collected from diverse geographical locations and rice varieties. Preliminary results indicated that each locu...
متن کاملIndependent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection by Magnaporthe grisea.
The phytopathogenic fungus Magnaporthe grisea elaborates a specialized infection cell called an appressorium with which it mechanically ruptures the plant cuticle. To generate mechanical force, appressoria produce enormous hydrostatic turgor by accumulating molar concentrations of glycerol. To investigate the genetic control of cellular turgor, we analyzed the response of M. grisea to hyperosmo...
متن کاملA Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection.
Cyclophilins are peptidyl prolyl cis-trans isomerases that are highly conserved throughout eukaryotes and that are best known for being the cellular target of the immunosuppressive drug cyclosporin A (CsA). The activity of CsA is caused by the drug forming a complex with cyclophilin A and inhibiting the calmodulin-dependent phosphoprotein phosphatase calcineurin. We have investigated the role o...
متن کاملA protein from the mold Aspergillus giganteus is a potent inhibitor of fungal plant pathogens.
A purified preparation of antifungal protein (AFP) from Aspergillus giganteus exhibited potent antifungal activity against the phytopathogenic fungi Magnaporthe grisea and Fusarium moniliforme, as well as the oomycete pathogen Phytophthora infestans. Under conditions of total inhibition of fungal growth, no toxicity of AFP toward rice protoplasts was observed. Additionally, application of AFP o...
متن کاملMolecular cloning and sequence analysis of a putative ras gene of the phytopathogenic fungus Botryotinia fuckeliana.
We solated and determined the complete nucleotide sequence of a ras gene from Botryotinia fuckeliana, the causal agent of gray mold disease. Furthermore, in two other phytopathogenic fungi, Magnaporthe grisea (rice blast fungus) and Colletotrichum lagenarium (an anthracnose fungus), ras gene homologs were identified by amplifying gene fragments in polymerase chain reactions. The putative ras ge...
متن کامل